Anti-Hyperglycemic and Lipid Lowering Activity of Trigonella foenum-graecum (Linn.) Leaves

Suman Kumar Mekap, Arun Kumar Panda, Prasana Kumar Panda, Saumya Ranjan Pani

Abstract


Objective: Trigonella foenum-graecum (Linn.) (T foenum-graecum) belonging to family Fabaceae collected from southern Odisha was aimed to appraise the anti-hyperglycemic and lipid lowering activity of ethanolic leaf extract (200 and 400 mg/kg b.w.) by two different models for Antidiabetic activity screening viz. Alloxan induced and Streptozotocin-Nicotinamide (STZ-NA) induced diabetic models and Triton (WR 1339) induced hyperlipidemic model in rats.

Method: The Antidiabetic activity of the extract was compared with standard drug Metformin (11.3 mg/kg of b.w.; p.o) and Gliclazide (25 mg/kg of b.w.; p.o) for Alloxan induced and Streptozotocin-Nicotinamide (STZ-NA) induced diabetic models respectively and Atorvastatin (7.2 mg/kg of b.w.; p.o) for Triton (WR 1339) induced hyperlipidemic model. The Blood glucose (GOD/POD method) and other biochemical parameters like total cholesterol, HDL cholesterol and triglyceride levels were estimated and very low density lipoprotein (VLDL) and low density lipoprotein (LDL) cholesterol were calculated.

Results: Treatment with T foenum graecum (400 mg/kg) to alloxan treated rats for 10 days significantly (p<0.001) attenuated the blood glucose level (141.30 ± 11.56 mg/dl) of test animals when compared to alloxan control (293.16 ± 7.32 mg/dl). Similarly, in STZ-NA induced diabetic model, significant (p<0.001) reduction in blood glucose level (152.61 ± 6.83 mg/dl) in test animals were observed, following the treatment of T foenum graecum (400 mg/kg) for 28 days. In Triton (WR 1339) induced hyperlipidemia (48 hour model), T foenum graecum (400 mg/kg) treatment exhibited significant (p<0.001) attenuation in serum total cholesterol (70.92 ± 3.46 mg/dl), triglyceride (125.67 ± 6.54 mg/dl) and LDL (10.42 ± 6.77 mg/dl) with significant (p<0.05) elevation in serum HDL (35.36 ± 5.32 mg/dl) levels. Statistical significance of data was assessed by One-way ANOVA followed with Tukey-Kramer Multiple comparison between different groups. The data obtained from the study suggested, the anti-diabetic and lipid lowering activities of T foenum graecum (Linn.) leaves.


Keywords


Antidiabetic, Lipid lowering, fenugreek, Triton

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