Analgesic activity of Lawsonia inermis leaves extract in swiss albino mice

Manivannan R., Aeganathan R.


Objective: Lawsonia inermis an important traditional medicinal plant used in various ailments and the indigenous plant as a medicament for treatment of various inflammatory conditions is well documented in literature. The analgesic activity was evaluated by hot-plate and acetic acid induced writhing methods.

Methods: The leaves of L. inermis were collected from the local area in and around Kumbakonam, Thanjavur District (India). About 1500 g of the powder was extracted using Soxhlet apparatus for 12 h using 5.0 L of methanol as solvent. The test extracts was administered intraperitoneally at a dose of 250 and 500 mg/kg while morphine sulfate (5 mg/kg) and diclofenac sodium (100 mg/kg) served as standards.

Results: All the extract displayed significant analgesic effect (P<0.05-0.001) in acetic acid and heat induced pain models in a dose dependent manner.

Conclusions: The results suggest that all the extracts of L. inermis possesses potent analgesic properties, which support its use in traditional medicine and suggesting that the plant should be further investigated for its pharmacological active natural products.


Analgesic activity, Hot-plate, Acetic acid induced writhing method, Lawsonia inermis

Full Text:



Kasture SB, Une HD, Sarveiyal VP, Pal SC, Kasture VS. Nootropic and anxiolytic activity of saponins of Albizzia lebbeck leaves. Pharmacology Biochemistry and Behavior. 2001;69:439-44.

Jallad KN, Jallad CE. Lead exposure from the use of Lawsonia inermis (Henna) in temporary paint-on-tattooing and hair dying. Science of the Total Environment. 2008;397:244-50.

Abdulmoneim MA. Evaluation of Lawsonia inermis Linn. (Sudanese Henna) leaf extract as an antimicrobial agent. Research Journal of Biological Sciences. 2007;2:417-23.

Dev S. A selection of prime ayurvadic plant drugs. Ancient- modern concordance. A Publisher, Delhi, India; 2006: 276-279.

Kirkland D, Marzin D. An assessment of the genotoxicity of 2-hydroxy-1, 4 naphthoquinone, the natural dye ingredient of Henna. Mutation Research. 2003;537:183-99.

Tripathi KD. Essentials of Medical Pharmacology. Edn 5, Jaypee Brothers Medical Publishers (P) Ltd; 2003: 453.

Hewitt DJ, Hargreaves RJ, Curtis SP, Michelson D. Challenges in analgesic drug development. Clinical Pharmacology &Therapeutics. 2009;86(4):447-50.

Hanson GR, Venturelli PJ, Fleckenstein AE. Drugs and Society, Jones and Bartlett, Boston, Mass, USA, 10th edition; 2009.

Baker JT, Borris RP, Carte B, Cordell GA, Soejarto DD. Natural product drug discovery and development: New perspective on international collaboration. Journal Natural Products. 1995;58(3):1325-57.

Litchfield JT, Wilcoxon F. A simplified method of evaluating dose-effect experiments. Journal of Pharmacology Experimental Therapy. 1949;96:99-133.

Eddy NB, Leimback D. Synthetic analgesic. II. Dithienyl butenylanddithienyl butyl amines. Journal of Pharmacology Experimental Therapy. 1953;107:385-93.

Koster R, Anderson M, De Beer EJ. Acetic acid for analgesics screening. Federation Proceeding. 1959;18:412-7.

Manivannan R, Aeganathan R, Prabakaran K. Anti-microbial and anti-inflammatory flavonoid constituents from the leaves of Lawsonia inermis. Journal of Phytopharmacology. 2015;4(4):212-6.

Vongtau HO, Abbah J, Mosugu O. Antinociceptive profile of the methanolic extract of Neorautanenia mitis root in rats and mice. Journal of Ethnopharmacol. 2004;92:317-24.

Duarte I, Nakamura M, Ferreira S. Participation of the sympathetic system in acetic acid-induced writhing in mice. Brazilian Journal of Medicinal and Biological Research. 1988;21(2):341.

Ronaldo AR, Mariana LV, Sara MT, Adriana BP, Steve P, Ferreira SH, et al. Involvement of resident macrophages and mast cells in the writhing nociceptive response induced by zymosan and acetic acid in mice. European Journal of Pharmacology. 2000;387:111-8.

Derardt R, Jougney S, Delevalcee F, Falhout M. Release of prostaglandins E and F in an algogenic reaction and its inhibition. European Journal of Pharmacology. 1980;51:17-24.

Kumara NKVMR. Identification of Strategies to Improve Research on Medicinal Plants Used in Sri Lanka. In WHO Symposium: University of Ruhuna. Galle, Sri Lanka. 2001:12–14.

Sengupta R, Sheorey SD, Hinge MA. Analgesic and anti-inflammatory plants: an updated review. International Journal of Pharmaceutical Sciences Review and Research. 2012;12(2):114–9.


  • There are currently no refbacks.

Copyright (c) 2016 Pharmaceutical and Biological Evaluations

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Creative Commons License


© Copyright 2018 - Pharmaceutical and Biological Evaluations