A study of the toxicity of dimethoate on human lymphocytes and the ameliorative effect of root extract of Oroxylum indicum (L.) Vent

Richa Soni, Asha Khanna, Mamta Gokhale, Dayashankar Gautam

Abstract


Objective: To study the toxicity of dimethoate on human lymphocytes and the ameliorative effect of root extract of Oroxylum indicum (L.) Vent.

Methods: Human peripheral blood lymphocytes were separated from human blood by HiseptmLSM1077. They were exposed to 20,40,60 and 100 mM concentrations of dimethoate for 1 hour and the DNA damage was assessed by fluorescent comet assay. The toxicity of the insecticide was also tested by MTT assay.

Results: It was found that the number of cells with no damage decreased with the rise in concentration of dimethoate and there was a dose dependent increase in the number of damaged cells (9-98%). In MTT assay the viability of control cells was taken as 100% and after 2 hours of exposure to various concentrations of dimethoate, there was dose dependent decrease in viability. The viability of treated cells increased after incubation with 50% of the root extract of Oroxylum indicum (L.) Vent. However, no ameliorative effect was observed when the treated lymphocytes were incubated with crude root extract.

Conclusions: Thus the experiment supports the fact that 50% of root extract of Oroxylum has an ameliorative effect on the lymphocytes which were subjected to the dimethoate toxicity.


Keywords


Ameliorative effect, Human lymphocytes, Oroxylum indicum, Root extract, Dimethoat

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References


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