<ArticleSet>
<Article>
<Journal>
<PublisherName>Pharmaceutical and Biological Evaluations</PublisherName>
<JournalTitle>Pharmaceutical and Biological Evaluations</JournalTitle>
<Issn>2394-0859</Issn>
<Volume>3</Volume>
<Issue>4</Issue>
<PubDate>
<Year>2016</Year>
<Month>08</Month>
<Day>01</Day>
</PubDate>
</Journal>
<ArticleTitle>A study of the toxicity of dimethoate on human lymphocytes and the ameliorative effect of root extract of Oroxylum indicum (L.) Vent</ArticleTitle>
<FirstPage>424</FirstPage>
<LastPage>430</LastPage>
<Language>EN</Language>
<AuthorList>
<Author>
<FirstName>Richa</FirstName>
<LastName>Soni</LastName>
<Affiliation>Department of Biotechnology, Pondicherry University, Pondicherry,. richasoni14@gmail.com</Affiliation>
</Author>
<Author>
<FirstName>Asha</FirstName>
<LastName>Khanna</LastName>
</Author>
<Author>
<FirstName>Mamta</FirstName>
<LastName>Gokhale</LastName>
</Author>
<Author>
<FirstName>Dayashankar</FirstName>
<LastName>Gautam</LastName>
</Author>
</AuthorList>
<History>
<PubDate>
<Year>2016</Year>
<Month>07</Month>
<Day>08</Day>
</PubDate>
<PubDate>
<Year>2016</Year>
<Month>07</Month>
<Day>22</Day>
</PubDate>
<PubDate>
<Year>2016</Year>
<Month>07</Month>
<Day>20</Day>
</PubDate>
</History>
<Abstract>Objective: To study the toxicity of dimethoate on human lymphocytes and the ameliorative effect of root extract of Oroxylum indicum (L.) Vent.Methods: Human peripheral blood lymphocytes were separated from human blood by HiseptmLSM1077. They were exposed to 20,40,60 and 100 mM concentrations of dimethoate for 1 hour and the DNA damage was assessed by fluorescent comet assay. The toxicity of the insecticide was also tested by MTT assay.Results: It was found that the number of cells with no damage decreased with the rise in concentration of dimethoate and there was a dose dependent increase in the number of damaged cells (9-98%). In MTT assay the viability of control cells was taken as 100% and after 2 hours of exposure to various concentrations of dimethoate, there was dose dependent decrease in viability. The viability of treated cells increased after incubation with 50% of the root extract of Oroxylum indicum (L.) Vent. However, no ameliorative effect was observed when the treated lymphocytes were incubated with crude root extract.Conclusions: Thus the experiment supports the fact that 50% of root extract of Oroxylum has an ameliorative effect on the lymphocytes which were subjected to the dimethoate toxicity.</Abstract>
<ObjectList>
<Object>
<Param>Ameliorative effect, Human lymphocytes, Oroxylum indicum, Root extract, Dimethoat</Param>
</Object>
</ObjectList>
<URLs>

Abstract

<Fulltext>
<pdf>http://onlinepbe.com/index.php/PBE/article/view/159/pdf</pdf>
</Fulltext>
</URLs>
</Article>
</ArticleSet>

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