Antihypertensive activity of Beta vulgaris on dexamethasone induced hypertension in rats

Dignesh Patel, Rupali Patil, Alkesh Patel

Abstract


Objective: Investigation of methanolic extract for detecting the presence of Betanin by phytochemical   analysis, to induce experimental hypertension and to carry out measurement of BP by invasive (direct) method (IBP) and vascular reactivity to various catecholamines after completion of treatment schedule.

Methods: The animals were divided into six main groups. Each group contains 5 animals. Group I received vehicle , group  II received dexamethasone injection (20 µg/kg/day, s.c.) for 14 days , group III received  extract of Beta vulgaris (100 mg/kg/day, p.o.) for 14  days , group IV received extract of Beta vulgaris (300 mg/kg/day, p.o.) for 14 days , group V received dexamethasone injection (20 ug/kg/day, s.c.) and extract  of  Beta  vulgaris (100 mg/kg/day, p.o.) for 14 days and group VI received dexamethasone (DEXA) injection (20 µg/kg/day, s.c.) and extract of  Beta  vulgaris (300 mg/kg/day, p.o.) for 14 days. After 14 days of dosing period body weight, ECG and changes in vascular reactivity to various catecholamines were recorded using Powerlab 4SP (AD Instrument, Australia).

Results: Animals treated with dexamethasone along with Beta vulgaris (100 and 300 mg/kg p.o. for 14 days)   showed a significant (p<0.05) decrease in heart rate compared to Dexamethasone treated group. Dexamethasone administered rats showed a significant elevation (p<0.05) in systolic blood pressure (SBP), vascular reactivity changes to Catecholamine as compared to control group. Beta vulgaris extract (100, 300 mg/kg/day, p.o.) treatment for 14 days  in  dexamethasone administered  rats significantly (p<0.05) reduced  systolic blood pressure, vascular reactivity changes to catecholamines as compared to dexamethasone administered group.

Conclusions: The methanolic-HCl extract of Beta vulgaris has antihypertensive activity in dexamethasone model.


Keywords


Hypertension, Dexamethasone, Beta vulgaris

Full Text:

PDF HTML XML

References


Chobanian AV, Bakris GL, Black HR. Seventh Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure. Hypertension. 2003;42:1206-52.

Vasan RS, Beiser A, Seshadri S, Larson MG, Kannel WB, D'Agostino RB, et al. Residual lifetime risk for developing hypertension in middle-aged women and men: The Framingham Heart Study. JAMA. 2002;287(8):1003-10.

Gupta R, Misra A, Pais P, Rastogi P, Gupta VP. Correlation of regional cardiovascular disease mortality in India with lifestyle and nutritional factors. Int J Cardiol. 2006;108(3):291-300.

Murray CJ, Lopez AD. Global mortality, disability, and the contribution of risk factors: Global Burden of Disease Study. Lancet. 1997;349:1436–42.

Kearney PM, Whelton M, Reynolds K, Muntner P, Whelton PK, He J. Global burden of hypertension: analysis of worldwide data. Lancet. 2005;365:217–23.

Harborne JB. Phytochemical methods A Guide to Modern Techniques of Plant Analysis, Published by Chapman and Hall, 3rd edition; 1997: 74.

Ganesan B, Anandan R. Protective effect of betaine on changes in the levels of lysosomal enzyme activities in heart tissue in isoprenaline-induced myocardial infarction in Wistar rats. Cell Stress and Chaperones. 2009;14:661–7.

Nossuli TO, Hayward R, Scalia R, Lefer AM. Peroxynitrite reduces myocardial infarct size and preserves coronary endothelium after ischemia and reperfusion in cats. Circulation. 1997;96:2317–24.

Hruza Z, Zweifach BW. Effect of Age on vascular reactivity to catecholamines in rats. J Gerontol. 1967;22(4):469-73.

Whelton A, White WB, Bello AE, Puma JA, Fort JG. Effects of celecoxib and rofecoxib on blood pressure and edema in patients > or = 65 years of age with systemic hypertension and osteoarthritis. Am J Cardiol. 2002;90:959–63.

Brashers VL, McCance KL. Structure and function of the cardiovascular and lymphatic systems. In: McCance KL, Huether SE, Brashers VL, Rote, NS, eds. Pathophysiology: The Biologic Basis for Disease in Adults and Children, 6th ed. Maryland Heights, MO: Mosby Elsevier; 2010: 1091-1141.

Lapa TA. Validacao de plants medicinais traditional. Reunion Annual Repronamed /Quimica Fina. Farmaceutica/CYTED-D. Asuncion, Paraguay; 1992: 23-27.

Camille J, Wallwork A, Dale A, Parks Geert BW. Xanthine oxidase activity in the dexamethasone-induced hypertensive rat. Microvascular Research. 2003;66:30–7.

Lee JU et al. Altered Nitric Oxide System in Cardiovascular and Renal Diseases. Chonnam Medical Journal. 2016;52.2:81–90.

Webb AJ et al. Acute blood pressure lowering, vasoprotective and anti-platelet properties of dietary nitrate via bioconversion to nitrite. Hypertension. 2008;51(3):784–90.




DOI: http://dx.doi.org/10.26510/2394-0859.pbe.2017.06

Refbacks

  • There are currently no refbacks.




Copyright (c) 2017 Pharmaceutical and Biological Evaluations

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.



Creative Commons License

 

© Copyright 2018 - Pharmaceutical and Biological Evaluations