Antifungal potential of improved crude extracts of Mitracarpus scaber (Zucc) against Candida guilliermondii and Candida parapsilosis

E. K. Kporou, C. Ibourahema, A. J. Ackah, S. Ouattara

Abstract


Objective: The present study was undertaken to evaluate antifungal potential of improved crude extracts of Mitracarpus scaber Zucc., an ivorian traditional medicinal plant against two species of clinical yeast isolates: Candida parapsilosis and Candida guilliermondii.

Methods: For evaluation of antifungal activity, double dilution method in slope tubes was used. Crude extracts of M. scaber were prepared by extracting with a blender in aqueous and hydroalcohol. In first; Aqueous (XAq) and Hydroalcoholic (X0) extracts were evaluated, and secondary the crude extract X0 was improved by partition in mixture of aqueous and hexanic solvent 50:50 v/v. Each dried phase was also examined. A phytochemical screening has been performed on most active extracts.

Results: In vitro antifungal activity showed that crude extract X0 was the most active with MIC = 6.25 mg/ml. Among the extracts obtained by partition from extract X0, extract X11 (Hexanic phase) was the better with lowest antifungal parameters MIC and MFC range between 1.562 mg/ml and 0.781 mg/ml and IC50 range between 0.73 mg/ml to 0.598 mg/ml. In addition, the most active extract contained only terpens and steroids.

Conclusions: Antifungal activity of M. scaber was improved by partition of an hydroalcoholic extract (X0) in mixture hexane-water 50:50 v/v. Hexanic phase (X11) was more active against C. parapsilosis and C. guilliermondii.


Keywords


Mitracarpus scaber, Antifungal, Performed extract

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References


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DOI: http://dx.doi.org/10.26510/2394-0859.pbe.2017.14

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