Antimicrobial activities of volatile oil compound from the rhizome of Zingiber officinale using solvent method extraction

Ugoh Sylvanus, Fatokun Olakunle, Olajide Olutayo, Kabiru Usman

Abstract


Objective: To characterize the chemical constituents and evaluate the antimicrobial activity of Zingiber officinale oil extracted with hexane.

Methods: The air dried powdered rhizome of Z. officinale was extracted with n- hexane. The antimicrobial activity of Z. officinale oil extract was tested on gram positive and gram negative bacteria.

Result: The extracted oil on GC-MS analysis resulted in the identification of 19 compounds, among which 13-Docosenoic acid was found to be the most abundant at 15.48 %. The preliminary antimicrobial screening of the oil from Z. officinale against four pathogens; Pseudomonas aeruginosa, Escherichia coli, Salmonella typhi and Staphylococcus aureus revealed that the mean zone diameter of inhibition (Z.O.I) is concentration dependent in which pseudomonas aeruginosa showed highest concentration at 100 %. The minimum inhibitory concentrations (MIC) values of the oil revealed these concentrations 12.5 %, 25 % 50 % and 100 % against the tested organisms respectively. The minimum bactericidal concentration (MBC) of the oil on S. aureus was recorded at 100% concentration, while E. coli, P. aeruginosa, and S. typhi were all resistant.

Conclusion: This study showed that the oil from Z. officinale may be used to manage some infectious diseases caused by the tested organisms.


Keywords


Zingiber officinale, Chemical constituents, Antimicrobial activities, Medicinal uses

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